Lapis ISSN 2398-2969

Staining techniques: New methylene blue

Synonym(s): NMB

Introduction

  • New methylene blue (NMB) is a supra vital stain that is now rarely used as a cytological stain.
  • It is an aqueous stain that allows instant examination after application to air dried cells.
  • Mixed with whole blood, RNA and DNA stain deep blue.
  • Reticulocyte count: RNA in cytosol of immature RBC stains deep blue when mixed with NMB.
  • Heinz bodies: denatured, precipitated hemoglobin seen as spherical pale blue structures found at the periphery of erythrocytes.

Requirements

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Procedure

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Outcomes

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Further Reading

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