Lapis ISSN 2398-2969

Staining technique: Wright-Giemsa

Synonym(s): Romanowsky stain

Contributor(s): Molly Varga, Sarah Pellett

Introduction

  • A Romanowsky-type stain   Staining technique: Romanowsky stains - overview  .
  • Romanowsky stains are neutral stains composed of a mixture of oxidized methylene blue (azure) dyes and Eosin Y.
  • The azures are basic dyes that bind acid nuclei and result in a blue to purple color.
  • The acid dyes: eosines are attracted to the alkaline cytoplasm, producing red coloration.
  • Wright-Giemsa stain is closely related to Wright's stain   Staining technique: Wright's  .

Uses

  • Differential staining of blood and hemoparasites.

Requirements

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Procedure

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Further Reading

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