Lapis ISSN 2398-2969

Staining technique: Wright-Giemsa

Synonym(s): Romanowsky stain

Contributor(s): Molly Varga, Sarah Pellett

Introduction

  • A Romanowsky-type stain   Staining technique: Romanowsky stains - overview  .
  • Romanowsky stains are neutral stains composed of a mixture of oxidized methylene blue (azure) dyes and Eosin Y.
  • The azures are basic dyes that bind acid nuclei and result in a blue to purple color.
  • The acid dyes: eosines are attracted to the alkaline cytoplasm, producing red coloration.
  • Wright-Giemsa stain is closely related to Wright's stain   Staining technique: Wright's  .

Uses

  • Differential staining of blood and hemoparasites.

Requirements

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Procedure

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Further Reading

Publications

Refereed papers

Other sources of information

  • Merck KGaA (2013)Cytodiagnosis Staining Methods.Merck Millipore Manual. Website:www.merckmillipore.com.
  • Dunn J K & Gerber K (2005)Diagnostic Cytology. In:BSAVA Clinical and Feline Clinical Pathology. 2nd edn. Eds: Villiers E & Blackwood L. BSAVA, Gloucester. pp 305-339.
  • Sink C A & Feldman B  F (2004)Laboratory Urinalysis and Haematology for the Small Animal Practitioner.New Tenton Media, Wyoming. ISBN: 978-1893441101.

Organisation(s)

  • Mary Pinborough, Pinmoore Animal Laboratory Services Ltd, The Coach House, Town House Barn, Clotton, Cheshire CW6 0EG, UK. Tel: +44 (0)1829 781855; Email:  sales@palsvetlab.co.uk ; Website:  www.palsvetlab.co.uk .


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