Lapis ISSN 2398-2969

Urinalysis: centrifuged deposit

Contributor(s): Michael Waters, Molly Varga, Vetstream Ltd

Overview

  • White and red blood cells, casts, crystals, bacteria and neoplastic cells can be seen on microscopic examination in certain diseases of the urinary tract, eg urinary tract infection   Cystitis  , urolithiasis   Urolithiasis  , acute renal failure.
  • Sediment examination is an essential part of urinalysis.

Sampling

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Tests

Methodologies

  • Centrifuge 5 ml at 500-3000 rpm for 5 minutes   Urinalysis: sediment 01  .
  • Decant supernatant.
    EitherAdd 1 drop of 'Sedistain' or a supravital stain such as Sternheimer-Malbin to the sediment.
    OrLeave 0.5 ml urine in centrifuge tube.
  • Resuspended sediment by vigorously tapping the centrifuge tube.
  • Transfer one drop of sediment to a microscope slide (via a pipette) and place a coverslip over it.
  • Lower the condenser on microscope to improve contrast.
  • Systematically examine entire specimen under the lower power objective, assessing quantity and type of sediment   Urinalysis: calcium oxalate crystal    Urinalysis: struvite crystal  .
  • Examine sediment under the high power objective to identify morphology of elements and to detect bacteria.

Availability

  • Can be performed in-house by most veterinary clinics.
  • All external laboratories

Technician (extrinsic) limitations

  • Following the same approach for every analysis results in consistent findings and allows the technician to develop experience.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references fromPubMed.
  • Benson K G, Paul-Murphy J (1999)Clinical Pathology of the domestic rabbit. Ed: Reavill D R.  Vet Clin Nirth Am2(3), 539-552.


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