Lapis ISSN 2398-2969

Hematology: platelet count

Synonym(s): Thrombocyte

Contributor(s): Michael Waters, Molly Varga, Vetstream Ltd

Overview

  • Formed from cytoplasmic remnants of megakaryocytes.
  • Participate in hemostasis: responsible for the initial hemostatic plug to prevent hemorrhage after initial injury.
  • Much smaller cells than red blood cells.
  • Round flate disks of cytoplasm with no nuclei.

Sampling

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Tests

Methodologies

QBC-V system
  • Not validated.

Manual count

  • With commercial blood dilution system + standard Neubauer chamber gives accurate platelet count.
  • Most automatic counts are also checked manually on a smear.
  • On Romansowsky or supravitally stained smear:
    • Count platelets and WBCs over same oil immersion field (10-30 platelets per x 1000 field in normal rabbit).
    • 6-7 platelets per field = ~100 x 10*9/l.
    • Check edges of smear for platelet clumping which   →   artificially low platelet counts.

Estimates only, considerable errors, but clinically useful.

Automated bood counter

  • Rapid but if platelet clumping occurs   →   low readings.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references fromPubMed.

Other sources of information

  • Campbell T W & Ellis C K (2007)Avian and Exotic Hematology and Cytology.3rd edn. Wiley-Blackwell. ISBN: 978-0813818115.


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