Felis ISSN 2398-2950

Staining techniques: Wright's stain

Synonym(s): Romanowskys stain

Contributor(s): Kathleen P Freeman, Karen L Gerber

Introduction

  • Romanowsky stains Staining techniques: Romanowsky-type stains are neutral stains composed of a mixture of oxidized methylene blue (azure) dyes and Eosin Y.
  • The azures are basic dyes that bind acid nuclei and result in a blue to purple color.
  • The acid dye, eosin, is attracted to the alkaline cytoplasm, producing red coloration.

Uses

  • Hematology and cytology.

Advantages

  • If 'hemofast' is used, it contains neutral methyl alcohol, ie does not contain acetone, therefore there is no need for prior fixing of the smeared slides.

Disadvantages

  • Must use distilled water for 'hemofast'.
  • Cannot use tap water since the latter contains interfering substances, particularly calcium which can cause precipitation of eosin to an insoluble calcium salt.

Requirements

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Procedure

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Aftercare

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Outcomes

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Further Reading

Publications

Refereed papers

Other sources of information

  • Lewis H B & Rebar A H (1979) Bone Marrow Evaluation in Veterinary Practice. Ralston Purina Company, USA. p 59.

Organisation(s)

  • Mascia Brunelli S.P.A.package insert for Hemofast, Viale Monza 272-20128 Milano, Italy. Tel: 02/25:51.641 - telex 330838 Mabios 1 - Telefax 02/2576428.


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