Felis ISSN 2398-2950
Staining techniques: Romanowsky-type stains
Synonym(s): Wright's, Giemsa, Diffquick
Contributor(s): Kathleen P Freeman, Karen L Gerber
- Romanowsky stains are neutral stains composed of a mixture of oxidized methylene blue (azure) dyes and Eosin Y.
- The azures are basic dyes that bind acid nuclei and result in a blue to purple color.
- The acid dye, eosin, is attracted to the alkaline cytoplasm, producing red coloration.
- Blood films, bone marrow examination, cytology.
- Readily available, easy to prepare, maintain and use.
- Nuclear and nucleolar detail not as good as Papanicolaou stain Staining techniques: Papanicolaou stain, but adequate to distinguish neoplasia from inflammation.
- pH sensitive therefore buffer and rinse water are critical.
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Other sources of information
- Dacia Sir J V & Lewis S M (2001) Practical Haematology. 6th edn, Longman Singapore Publishers Pte Ltd, pp 50-61.
- Harvey J W (2001) Atlas of Veterinary Hematology. Blood and Bone Marrow of Domestic Animals. W B Saunders, p 11.
- Bain B J (1989) Blood Cells, A Practical Guide. Gower Medical Publishing, pp 810.
- Hall R & Malia R (1984) Medical Laboratory Haematology. Butterworth & Co (publishers) Ltd, pp 132- 136.
- Pratt P W Laboratory Procedures For Veterinary Technicians. 3rd edn. Mosby, p 547.