Felis ISSN 2398-2950

Staining techniques: Perl's Prussian Blue/Safranin

Synonym(s): Perls, Prussian blue

Contributor(s): Kathleen P Freeman, Karen L Gerber

Introduction

  • Hemosiderin combines with potassium ferrocyanide to form a colored pigment, potassium ferric ferrocyanide.
  • Iron-positive material stains blue, rest of cell and background stain dark pink (contrast).

Uses

  • Bone marrow - evaluate iron stores, helps to classify cause of anemia Anemia: overview.

Anemia due to iron deficiency, has decreased bone marrow storage of iron. Anemia due to hemolysis or decreased production has increased bone marrow iron stores.

  • Siderocytes/sideroblasts RBC with iron that appear as focal areas of basophilic stippling.
  • Sideroleukocyte - leukocytes containing iron-positive inclusions.
  • Hemosiderin - in macrophages in TW/BAL or body cavity fluids or other specimens.

Advantages

  • 1% acidic potassium ferrocyanide solution only has a shelf life of a few hours, therefore must always be made fresh.
  • Biohazard since need to store strong acids in clinic/laboratory.
  • 2% Potassium ferrocyanide must be kept in a brown bottle, stored in a dark cupboard to keep it stable.

Requirements

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Procedure

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Aftercare

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Further Reading

Publications

Refereed papers

Other sources of information

  • Harvey J W (2001) Atlas of Veterinary Hematology. Blood and Bone Marrow of Domestic Animals. W B Saunders. pp 14-15.
  • Lewis  H B & Rebar A H (1979) Bone marrow Evaluation in Veterinary Practice. Ralston Purina Company, ISB 78-68556,  pp 64-65.


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