Felis ISSN 2398-2950
Staining techniques: Leishman's stain
Contributor(s): Kathleen P Freeman, Karen Gerber
- A neutral stain for blood smears devised by a British surgeon,W B Leishman (1865-1926).
- Double staining compound of Methylene blue and Eosin in alcohol, which is usually diluted and buffered before use.
- It stains the different components of blood in a range of shades between red and blue.
- Stains WBC, RBC and platelets.
- Referral to laboratory or human medical institution.
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Other sources of information
- Dacia Sir J V & Lewis S M (2001) Practical Haematology. 6th edn, Longman Singapore publishers Pte Ltd, pp 50-61.
- Bain B J (1989) Blood Cells, A Practical Guide. Gower Medical Publishing, pp 810.
- Hall R & Malia R (1984) Medical Laboratory Haematology. Butterworth & Co (publishers) Ltd, pp 132-136.