Felis ISSN 2398-2950

Rose-Bengal staining

Contributor(s): Dennis E Brooks, Peter Renwick, David Williams

Introduction

  • Rose-Bengal stain binds to dead/degenerating cells and mucus.
  • Rose-Bengal thus shows mucin layer and/or epithelial cell erosion where stroma is not exposed and would thus be fluorescein-negative.
  • Can be used to detect mucin layer defects and early corneal damage in keratoconjunctivitis sicca (KCS).
  • The test is most useful for detecting early dendritic ulcers in feline herpesvirus keratitis Cornea: herpesvirus keratitis. It is otherwise rarely used.

Uses

Advantages

  • Simple test to perform.
  • Rapid results.

Disadvantages

Rose Bengal stain is irritant to the human eye, but generally tolerated by cats.
  • The result may be misleading - stain deposits may occur.

Requirements

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Preparation

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Procedure

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Outcomes

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Further Reading

Publications

Refereed papers

Other sources of information

  • Petersen-Jones S & Crispin S (2002) BSAVA Manual of Small Animal Ophthalmology. 2nd edn. British Small Animal Veterinary Association. ISBN 0 905214 54 4


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