Felis ISSN 2398-2950

Fluorescein test

Contributor(s): Ralph Hamor, Peter Renwick, David Williams

Introduction

  • Stains precorneal tear film and exposed corneal stroma (strongly hydrophilic).
  • Fluorescein will not penetrate intact corneal epithelium (high lipid content hydophobic).
  • In deep ulcers Ulcerative keratitis will show reduced staining because of thinner layer of stroma available for dye binding.
  • Does not stain exposed Descemet's membrane.
  • Stains corneal epithelial defects green and conjunctival ulcers yellow-orange, although the value of the latter property is reduced by poor contrast.

Uses

  • Check integrity of corneal epithelium, eg corneal ulceration Ulcerative keratitis.
  • Test of nasolacrimal duct patency.
  • Intravenous use (fluorescein angiography) to detect retinal vascular abnormalities, inflammatory lesions and intra-ocular neoplasms.

Advantages

  • Easy test to perform (with exception of intravenous use).
  • Minimal equipment required.
  • Rapid result.
  • Provides clinically important information.

Disadvantages

  • May obtain false impression of staining if eye is not irrigated with saline after fluorescein is applied.

Requirements

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Preparation

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Procedure

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Outcomes

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Further Reading

Publications

Refereed papers

Other sources of information

  • Gelatt K N (1999) Ocular Examination and Diagnostic techniques. In: Veterinary Ophthalmology. 3rd edn. Lippincott, Williams & Wilkins, Philadelphia.
  • Severin G A (1996) Examination of the Eye. In: Severin's Veterinary Ophthalmology Notes. 3rd edn.
  • Petersen-Jones S & Crispin S (2002) BSAVA Manual of Small Animal Ophthalmology. 2nd edn. British Small Animal Veterinary Association. ISBN 0 905214 54 4


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