ISSN 2398-2950      

Progesterone assay

ffelis

Overview

  • Progesterone responsible for secretory changes in endometrium. 
  • Secreted by the corpus luteum, placenta, testis (Leydig cells) and adrenal cortex.  
  • Progesterone concentrations are very low in proestrus. 
  • Rise to peak about 10 days after luteinizing hormone (LH) surge. 
  • Rises steadily until day ~ 21 post-ovulation:
    • In non-pregnant diestrus queens, it begins to decline shortly thereafter remaining partially elevated for 35-40 days. 
    • In pregnant queens, it plateaus at 30 days of gestation and slowly decline until baseline levels are reached by 63-65 days of gestation.

Sampling

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Tests

Methodologies

  • RIA  →  accurate, time consuming, slow turnaround time  
  • Fluorescence enzyme immunoassays  → rapid, accurate, user friendly interface; several equipment available for in-house use. 
  • In-house ELISA kits à semi-quantitative, fast, inexpensive. 

Availability

  • Widely available.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMed Resource and PubMed.
  • Mitacek M G, Stornelli M C, Praderio R G, de la Sota R L, Stornelli M A (2015) Ultrasonographic and progesterone changes during Days 21 to 63 of pregnancy in queens. Theriogenology 84(7), 1131-1141 PubMed
  • Siemieniuch M J, Jursza E, Szostek A Z, Skarzynski D J, Boos A, Kowalewski M P (2012) Steroidogenic capacity of the placenta as a supplemental source of progesterone during pregnancy in domestic cats. Reprod Biol Endocrinol 10(1), 1-10 PubMed
  • England G C (1997) Confirmation of ovarian remnant syndrome in the queen using hCG administration. Vet Rec 141(12), 309-310.
  • Swanson W F et al(1995) Relationship of circulating steroid hormones, luteal luteinizing hormone receptor and progesterone concentration, and embryonic mortality during early embryogenesis in the domestic cat. Biol Reprod 53(5), 1022-1029.

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