Felis ISSN 2398-2950
Fecal analysis: hemolytic E. coli
Contributor(s): Helen Milner, James Simpson
- E. coli Escherichia coli is a normal commensal found in the gastrointestinal tract.
- Presence of E. coli on routine culture is therefore common.
- Pathological strains do occur and must be distinguished from pathogenic strains.
- Must demonstrate:
- Pathogenic strains include:
- Enterotoxigenic E.coli (ETEC).
- Enteropathogenic E.coli (EPEC).
- Cytotoxic E.coli (VTEC/STEC).
- Enteroaggressive E. coli (EAEC).
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- Submit fecal samples for culture. Check with the receiving laboratory before requesting typing.
- The receiving laboratory should be notified of a potentially pathogenic E. coli case.
- Serotyping and PCR for determination of pathogenic strains of E. coli.
- Intestinal biopsy samples for histopathology to confirm attachment of E. coli to mucosal cells.
Dispose of consumables and fecal material following Health and Safety regulations.
Technique (intrinsic) limitations
- Many microbiology laboratories will not have the facility to type E. coli.
- Requires specialist laboratory examination in UK.
- May fail to differentiate commensal from pathogenic E. coli.
Other sources of information
- Manual of Small Animal Clinical Pathology. (1998) Davidson M, Else R & Lumsden J (eds), BSAVA.
- Clinical Textbook for Veterinary Technicians. (1998) 4th edn McCurnin D M (ed), W B Saunders.
- BSAVA Manual of Canine and Feline Gastroenterology. (1997) Thomas D A et al (eds), Iowa State University Press.
- Laboratory Procedures for Veterinary Technicians. (1997) 3rd edn, Pratt P W (ed), Mosby.