ISSN 2398-2950      

Fecal analysis: hemolytic E. coli

ffelis

Overview

  •  E. coli  Escherichia coli is a normal commensal found in the gastrointestinal tract.
  • Presence of E. coli on routine culture is therefore common.
  • Pathological strains do occur and must be distinguished from pathogenic strains.
  • Must demonstrate:
  • Pathogenic strains include:
    • Enterotoxigenic E.coli (ETEC).
    • Enteropathogenic E.coli (EPEC).
    • Cytotoxic E.coli (VTEC/STEC).
    • Enteroaggressive E. coli (EAEC).

Sampling

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Tests

Methodologies

  • Submit fecal samples for culture. Check with the receiving laboratory before requesting typing.
  • The receiving laboratory should be notified of a potentially pathogenic E. coli case.
  • Serotyping and PCR for determination of pathogenic strains of E. coli.
  • Intestinal biopsy samples for histopathology to confirm attachment of E. coli to mucosal cells.
    Dispose of consumables and fecal material following Health and Safety regulations.

Technique (intrinsic) limitations

  • Many microbiology laboratories will not have the facility to type E. coli.
  • Requires specialist laboratory examination in UK.
  • May fail to differentiate commensal from pathogenic E. coli.

Further Reading

Publications

Refereed papers

Other sources of information

  • Manual of Small Animal Clinical Pathology (1998) Eds: Davidson M, Else R & Lumsden J. BSAVA, UK.
  • Clinical Textbook for Veterinary Technicians (1998) 4th edn. Ed: McCurnin D M. W B Saunders.
  • BSAVA Manual of Canine and Feline Gastroenterology (1997) Eds: Thomas D A et al. Iowa State University Press, USA.
  • Laboratory Procedures for Veterinary Technicians (1997) 3rd edn. Ed: Pratt P W. Mosby.

MEMBER BENEFIT

Can’t find what you’re looking for?

We have an ever growing content library on Vetlexicon so if you ever find we haven't covered something that you need please fill in the form below and let us know!

 
 
 
 

To show you are not a Bot please can you enter the number showing adjacent to this field

 Security code