Felis ISSN 2398-2950

Blood biochemistry: vitamin B12

Synonym(s): B12, cobalamin

Contributor(s): Craig Ruaux, Kenneth Simpson

Overview

  • Absorbed in association with intrinsic factor   →   decreased IF   →   decreased absorption of clot   →   low blood concentration.
  • Absorbed in ileum   →   malabsorption results in low levels in blood.
  • Utilized by small intestinal bacteria   →   GI obstruction   →   bacterial growth   →   bacterial overgrowth reduces levels.

Sampling

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Tests

Methodologies

  • Radioimmunoassay.
  • Chemiluminescent immunoassay.
  • Bioassays.

Availability

  • Specialist laboratory - must use assay that boils serum/plasma.
  • Canine and feline serum may contain binding proteins that need to be denatured and absorbed before measurement via a "charcoal boil" step. Human laboratories may not necessarily carry out this step, by preference use a laboratory that routinely measures vitamin B12 in veterinary species.

Technician (extrinsic) limitations

  • Specialized.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMed Resource and PubMed.
  • Reed N, Gunn-Moore D & Simpson K (2007) Cobalamin, folate and inorganic phosphate abnormalities in ill cats. J Fel Med Surg 9, 278-288 PubMed.
  • Simpson K W, Fyfe J, & Cornetta A et al(2001) Subnormal concentrations of serum cobalamin (Vitamin B12) in cats with gastrointestinal disease. JVIM 15, 26-32.

Other sources of information

  • Simpson J W (1998) Gastrointestinal system and exocrine pancreas. In: Manual of Small Animal Clinical Pathology. Davidson M, Else R & Lumsden J (eds). BSAVA, Cheltenham.
  • Bush B M (1991) Interpretation of Laboratory Results. Blackwell Scientific Publication, Oxford.


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