Exotis ISSN 2398-2985

Reptiles

Staining technique: Wright-Giemsa stain

Synonym(s): Romanowsky stain

Contributor(s): Vetstream Ltd, Lesa Thompson

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Introduction

  • A Romanowsky-type stain.
  • Romanowsky stains are neutral stains composed of a mixture of oxidized methylene blue (azure) dyes and Eosin Y.
  • The azures are basic dyes that bind acid nuclei and result in a blue to purple color.
  • The acid dyes: eosines are attracted to the alkaline cytoplasm, producing red coloration.
  • Wright-Giemsa stain is closely related to Wright's stain.

Uses

  • Differential staining of blood and hemoparasites.

Requirements

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Procedure

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Outcomes

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Further Reading

Publications

Refereed papers

  • Recent references from PubMed and VetMedResource.
  • Alleman A R I, Jacobson E R & Raskin R E (1999) Morphologic, cytochemical staining, and ultrastructural characteristics of blood cells from eastern diamondback rattlesnakes (Crotalus adamanteus). Am J Vet Res 60 (4), 507-514 PubMed.
  • Mateo M R, Roberts E D & Enright F M (1984) Morphologic, cytochemical, and functional studies of peripheral blood cells of young healthy American alligators (Alligator mississippiensis). Am J Vet Res 45 (5), 1046-1053 PubMed.

Other sources of information

  • Campbell T W (2015) Stains and Solutions Used in Hematology and Cytology. In: Exotic Animal Hematology and Cytology. 4th edn. Wiley-Blackwell, USA. pp 381

Reproduced with permission from Bonnie Ballard & Ryan Cheek: Exotic Animal Medicine for the Veterinary Technician © 2017, published by John Wiley & Sons.


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