ISSN 2398-2985      

Urinalysis: centrifuged deposit

4ferrets

Overview

  • Microscopic examination of the urine sediment can reveal a variety of substances, including white and red blood cells, sloughing of tissue (debris), crystals, casts, bacteria, cells from the urinary tract or neoplastic cells.
  • Depending on the type of sediment, the cause may vary considerably. The most common cause of sediment in the urine is a urinary tract infection.
  • Sediment examination is an essential part of urinalysis and is often used to confirm the findings of other tests or add information to achieve a diagnosis.

Sampling

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Tests

Methodologies

  • Centrifuge 5 ml at 3000 rpm for 6 minutes.
  • Decant supernatant.
    • Either Add 1 drop of 'Sedistain' or a supravital stain such as Sternheimer-Malbin to the sediment.
    • Or Leave 0.5 ml urine in centrifuge tube.
  • Resuspended sediment by vigorously tapping the centrifuge tube.
  • Transfer one drop of sediment to a microscope slide (via a pipette) and place a coverslip over it.
  • Lower the condenser on microscope to improve contrast.
  • Systematically examine entire specimen under the lower power objective, assessing quantity and type of sediment.
  • Examine sediment under the high power objective to identify morphology of elements and to detect bacteria.

Availability

  • Can be performed in-house by most veterinary clinics.
  • All external laboratories.

Technician (extrinsic) limitations

  • Following the same approach for every analysis results in consistent findings and allows the technician to develop experience.

Result Data

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Further Reading

Publications

Refereed Papers

  • Recent references from PubMed and VetMedResource.
  • Lloyd M (2002) Veterinary care of ferrets: 1. Clinical examination and routine procedures. In Practice 24 (2), 90-95

Other sources of information

  • Chitty J (2009) Ferrets: physical examination and emergency care. In: BSAVA Manual of Ferrets and Rodents. Eds: Keeble E & Meredith A. British Small Animal Veterinary Association, UK. pp 205-218.
  • Fisher P G (2009) Ferrets: urogenital and reproductive system disorders. In: BSAVA Manual of Ferrets and Rodents. Eds: Keeble E & Meredith A. British Small Animal Veterinary Association, UK. pp 291-302.

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