Equis ISSN 2398-2977

Cornea: fluorescein dye test

Contributor(s): Keith Barnett, Dennis E Brooks

Introduction

  • Fluorescein is a water soluble dye which is unable to penetrate the lipid containing corneal or conjunctival eipthelium unless there is a break in its integrity. This allows the identification of damage in these structures which otherwise might be difficult to detect.

Uses

  • All cases with signs of corneal disease and/or ocular pain especially ulceration .
  • As part of complete ophthalmologic examination .
  • Check patency of naso-lacrimal drainage apparatus   Nasolacrimal duct: obstruction - acquired  (not always reliable).

Advantages

  • Cheap, easy to use, readily available.
  • Highly sensitive to epithelial breaks.
  • Well tolerated by eye.

Disadvantages

  • Fluorescein inpregnated strips.
  • Single use applicators are available but care is used to avoid contamination of old solutions byPseudomonasspp   Pseudomonas spp  .
  • Easily stains other articles including client's clothing (is water soluble and will wash out).

Requirements

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Preparation

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Procedure

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Further Reading

Publications

Refereed papers

Other sources of information

  • Brooks D E (1999)Equine Ophthalmology.In:Veterinary Ophthalmology.Ed: Gelatt K N. 3rd edn. 1053-1116.


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