Equis ISSN 2398-2977

Urine: centrifuged deposit

Contributor(s): Annalisa Barrelet, Frank Taylor

Overview

Sampling

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Tests

Methodologies

  • Centrifuge 5 ml at 500-3000 rpm for 5 min   Urine: aspirate  .
  • Decant supernatant.
    EitherAdd 1 drop of sedistain or a supravital stain such as Sternheimer-Malbin to the sediment.
    OrLeave 0.5 ml urine in centrifuge tube.
  • Resuspended sediment by vigourously tapping the centrifuge tube.
  • Transfer one drop of sediment to a microscope slide (via a pipette) and place a coverslip over it.
  • Lower the condenser on microscope to improve contrast.
  • Systematically examine entire specimen under the lower power objective, assessing quantity and type of sediment .
  • Examine sediment under the high power objective to identify morphology of elements and to detect bacteria .

Availability

  • Can be performed in-house by most veterinary clinics.
  • All external laboratories

Technician (extrinsic) limitations

  • Following the same approach for every analysis results in consistent findings and allows the technician to develop experience.

Result Data

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Further Reading


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