Equis ISSN 2398-2977

Peritoneal fluid: cytology

Contributor(s): Annalisa Barrelet, Kathleen P Freeman, Rose Raskin, Frank Taylor

Overview

  • Cell characteristics vary in septic conditions and with neoplasia.
  • Cytology enables characterization of abdominal effusion, and positive diagnosis in cases of exfoliating neoplasia.

Sampling

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Tests

Methodologies

  • Smear as for blood smear   Blood: smear preparation 01    Blood: smear preparation 02  or alternatively with squash technique.
  • Centrifuge at 1000 rpm for 5 minutes, pour off supernatant and re-suspend.
  • Cytospin centrifuge.
  • Stain with Romanowsky-type stains such as Wright's or Diff-Quik-like.

Technician (extrinsic) limitations

  • Reactive cells may be difficult to differentiate from dysplastic cells and neoplastic cells especially when there is significant inflammation.

Result Data

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Further Reading

Publications

Other sources of information

  • Cowell R L & Tyler R D (1992) In:Cytology and Hematology of the Horse.Mosby, St. Louis. pp 121-151.


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