Equis ISSN 2398-2977

Endocrine: parathyroid hormone assay

Synonym(s): PTH assay

Contributor(s): Kathleen P Freeman, Ramiro Toribio, Han van der Kolk

Overview

  • There are several assays to measure equine parathyroid hormone (PTH) concentrations in serum or plasma.
  • These assays determine either fragments of PTH or intact PTH (i-PTH).
  • The assays are valuable to assess primary or secondary alterations of parathyroid gland function that can result in an abnormal mineral metabolism.

Sampling

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Tests

Methodologies

  • Rat and human PTH immunoradiometric (IRMA) assays can be used to detect equine intact PTH (i-PTH).
  • Human immunochemiluminometric assays (ICMA) have been used to detect equine i-PTH.
  • New human IRMA to detect whole PTH (w-PTH) can also be used in horses.

Availability

  • Limited; only available in a few veterinary commercial/reference/university laboratories.
  • May be available via human hospitals.
  • Preference must be given to intact PTH assays, rather than to C-terminal or N-terminal PTH assays which do not detect the intact peptide.

Validity

Sensitivity

  • Check with laboratory to determine that their method has been validated for horses and that reference interval/guidelines for interpretation are available.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references fromPubMedpublished during the last 12 months.
  • Estepa J C et al(2003)Validation and clinical utility of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone in the horse. Equine Vet J35(3), 291-295PubMed.
  • Toribio R E et al(2001)Comparison of serum parathyroid hormone and ionized calcium and magnesium concentrations and fractional urinary clearance of calcium and phosphorus in healthy horses and horses with enterocolitis. Am J Vet Res62(6), 938-947PubMed.
  • Estepa J C et al(1998)Measurement of parathyroid hormone in horses. Equine Vet J30(6), 476-481PubMed.
  • Van der Kolk J H, Geelen S N, Jonker F H, Pycock J F & Koeman J P (1998)Hypertrophic osteopathy associated with ovarian carcinoma in a mare. Vet Rec143(6), 172-173PubMed.
  • Cooper S R et al(1995)Effects of dietary cation-anion balance on blood pH, acid-base parameters, serum and urine mineral levels and parathyroid hormone (PTH) in weaning horses. J Equine Vet Sci15(10), 417-420.
  • Elfers R S, Bayly W M et al(1985)Alterations in calcium, phosphorus and C-terminal parathyroid hormone levels in equine acute renal disease. Cornell Vet76(3), 317-329PubMed.
  • Brobst D F, Bayly W M et al(1982)Parathyroid hormone evaluation in normal horses and horses with renal failure. J Equine Vet Sci2(5), 150-157.

Other sources of information

  • Toribio R E (2004)Calcium Disorders.In: Equine Internal Medicine. Eds: Reed S M, Bayly W M & Sellon D C. 2nd edn. Saunders, St Louis. pp 1295-1327.
  • Fenger C K (1998)Disorders of Calcium Metabolism.In: Equine Internal Medicine.Eds: Reed S M & Bayly W M. Philadelphia: W B Saunders Co. pp 925-934.


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