Equis ISSN 2398-2977

CSF: cell count and differential

Contributor(s): Frank Andrews, Annalisa Barrelet, Caroline Hahn, Rose Raskin

Overview

  • Cell numbers generally increase in acute inflammation and some other conditions.
  • Differential counts may help in diagnosis.

Sampling

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Tests

Methodologies

Total cell count - hemocytometer
  • If using undiluted CSF, use a microhematocrit tube to fill both sides of the hemocytometer: Number of red and white cells counted in 5 of the large squares on each side for a total of 10 large squares. Cells counted = cells/ul.

Differential cell concentration

  • Sedimentation.
  • Centrifugation.
  • Membrane filtration.
  • Cytocentrifugation.

Differential cell count

  • Romanowsky-type stained smear.

Availability

  • Widely available.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references fromPubMedpublished during the last 12 months.
  • Johnson P J, Constantinescu G M (2000)Collection of cerebrospinal fluid in horses. Equine Vet Educ12, 7-12.
  • Johnson P J, Constantinescu G M (2000)Analysis of cerebrospinal fluid in horses. Equine Vet Educ12, 13-17.

Other sources of information

  • MacWilliams P S (1992) Cytology and Hematology of the Horse.Mosby, St. Louis. pp 163-171.


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