Equis ISSN 2398-2977
Contributor(s): Robert Shull
- Test for blood compatibility between donor and recipient horses.
- Preferably test before any transfusion.
- Essential for second and subsequent donations.
- Major crossmatch = recipients serum against donor red cells.
- Minor crossmatch = recipients red cells against donor serum.
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- Samples from donor and recipient should be prepared in the same way but should be carefully labeled to avoid confusion.
To prepare washed red cells
- Place 5 ml of saline in a sterile centrifuge tube.
- Place a glass rod into the clotted blood and transfer the rod carrying red cells to the saline to give a weak suspension of red blood cells.
- Centrifuge suspension for 2-3 minutes.
- Pour off supernatant.
- Resuspend cells in 5 ml of clean saline.
- Repeat centrifugation.
- Repeat washing of red cells at least 5 times.
- After the last wash resuspend cells in 1 or 2 drops of saline.
- This provides the final suspension of washed red blood cells for the test.
To prepare serum
- Centrifuge clotted blood for 2-3 minutes or until serum is clear.
- Clean 2 glass slides.
- Place on white paper.
- Label slides, one for donor serum, the other recipient serum.
- Place 2 separate drops of the appropriate serum on each slide.
- Add 1 drop of washed red cells to each drop of serum.
- To the donor serum add 1 drop of donor cells (this acts as a control).
- To the other drop of donor serum on that slide add one drop of recipient cells (this is the test).
- Likewise to the recipient serum add one drop each of donor cells (test) and recipient serum (control).
- Label carefully.
- Agitate slide carefully to mix red cells evenly with the serum.
- Incubate at room tempetaure for 10-15 minutes (cover with Petri dish to prevent evaporation).
- Even sedimentation of red cells in all 4 spots, ie donor serum with donor and recipient cells and recipient serum with donor and recipient cells.This is a fully compatible crossmatch.
- Even sedimentation except in donor serum with recipient cells which show uneven hemagglutination.This is an incompatible crossmatch.
- Even sedimentation in control spots only. Hemoagglutination in both test spots.This is a totally incompatible crossmatch.