ISSN 2398-2977      

Blood: cross-matching

pequis
Contributor(s):

Robert Shull


Overview

  • Test for blood compatibility between donor and recipient horses.
  • Preferably test before any transfusion.
  • Essential for second and subsequent donations.
  • Major crossmatch = recipients serum against donor red cells.
  • Minor crossmatch = recipients red cells against donor serum.

Sampling

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Tests

Methodologies

  • Samples from donor and recipient should be prepared in the same way but should be carefully labeled to avoid confusion.
    To prepare washed red cells
  • Place 5 ml of saline in a sterile centrifuge tube.
  • Place a glass rod into the clotted blood and transfer the rod carrying red cells to the saline to give a weak suspension of red blood cells.
  • Centrifuge suspension for 2-3 minutes.
  • Pour off supernatant.
  • Resuspend cells in 5 ml of clean saline.
  • Repeat centrifugation.
  • Repeat washing of red cells at least 5 times.
  • After the last wash resuspend cells in 1 or 2 drops of saline.
  • This provides the final suspension of washed red blood cells for the test.
    To prepare serum
  • Centrifuge clotted blood for 2-3 minutes or until serum is clear.
    Compatibility test
  • Clean 2 glass slides.
  • Place on white paper.
  • Label slides, one for donor serum, the other recipient serum.
  • Place 2 separate drops of the appropriate serum on each slide.
  • Add 1 drop of washed red cells to each drop of serum.
  • To the donor serum add 1 drop of donor cells (this acts as a control).
  • To the other drop of donor serum on that slide add one drop of recipient cells (this is the test).
  • Likewise to the recipient serum add one drop each of donor cells (test) and recipient serum (control).
  • Label carefully.
  • Agitate slide carefully to mix red cells evenly with the serum.
  • Incubate at room tempetaure for 10-15 minutes (cover with Petri dish to prevent evaporation).
  • Read.
    Results
  • Even sedimentation of red cells in all 4 spots, ie donor serum with donor and recipient cells and recipient serum with donor and recipient cells.This is a fully compatible crossmatch.
  • Even sedimentation except in donor serum with recipient cells which show uneven hemagglutination.This is an incompatible crossmatch.
  • Even sedimentation in control spots only. Hemoagglutination in both test spots.This is a totally incompatible crossmatch.

Further Reading

Publications

Refereed papers

MEMBER BENEFIT

Can’t find what you’re looking for?

We have an ever growing content library on Vetlexicon so if you ever find we haven't covered something that you need please fill in the form below and let us know!

 
 
 
 

To show you are not a Bot please can you enter the number showing adjacent to this field

 Security code