Equis ISSN 2398-2977

Blood: biochemistry - total bilirubin

Synonym(s): Direct and indirect bilirubin, conjugated and unconjugated bilirubin

Contributor(s): Annalisa Barrelet, Kathleen P Freeman, Frank Taylor

Overview

  • Peripheral breakdown of red blood cells and myoglobin   →   unconjugated (indirect) bilirubin   →   bound to albumin   →   transported to liver   →   conjugated with glucuronic acid   →   excreted in bile.
  • Jaundice, with an increase in total bilirubin, may be primarily due to an increase in direct, or indirect bilirubin or both.

Sampling

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Tests

Methodologies

  • Van den Bergh method.
  • Colorimetric.
  • Measure [total bilirubin] and [direct bilirubin]   →   [indirect bilirubin] = difference (total bilrubin - direct bilirubin = indirect bilirubin). Indirect bilirubin liberated by a detergent.

Availability

  • Routine test, widely available.

Technique (intrinsic) limitations

  • Interpret results in conjunction with other laboratory results and clinical signs.

Technician (extrinsic) limitations

  • Routine test.

Result Data

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Further Reading

Publications

Refereed papers

  • Engleking L R & Paradis M R (1987)Evaluation of hepatobiliary disorders in the horse. Vet Clin North Am Equine Pract3(3), 563-583.
  • Ogilvie G K, Engleking L R & Anwe M S (1985)Effects of plamsa sample storage of on blood ammonia, bilirubin and urea nitrogen concentrations - cats and horses. Am J Vet Res46(12), 2619-2622.

Other sources of information

  • Barton M H & Morris D D (1998)diseases of the Liver.In: Equine Internal Medicine.Eds: S M Reed & W M Bayly. Philadelphia: W B Saunders Co.
  • Kaneko J J, Harvey J W & Bruss M L(1997) EdsClinical Biochemistry of Domestic Animals.5th edn. Academic Press, Boston.

Organisation(s)

  • Veterinary commercial laboratories, State diagnostic laboratories or Veterinary College laboratories.


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