Equis ISSN 2398-2977

Blood: biochemistry - intestinal alkaline phosphatase

Synonym(s): SAP, IAP, SIAP

Contributor(s): Annalisa Barrelet, Kathleen P Freeman, Frank Taylor

Overview

  • Isoenzyme of serum alkaline phosphate (SAP or AP) derived from intestinal brush border cells.
  • Cellular disruption results in increased levels in blood.
  • Analytical methods may vary, but may measure SAP at the alkaline pH 10.5. Intestinal alkaline phosphate (IAP) is measured at pH 9.5 and is not inhibited by the aromatic amino acid phenylalanine. IAP is therefore calculated by the subtraction method of enzymatic activity at pH 9.5 with and without phenylalanine. Not commonly offered as a veterinary chemistry test in the USA.

Sampling

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Tests

Methodologies

  • Colorimetric method based on binding to bromocresol green.
  • Other dyes may produce erroneous results with equine specimens.

Availability

  • Widely available.

Technique (intrinsic) limitations

  • Only useful in conjunction with other laboratory and clinical findings.
  • May be falsely elevated by hemolysis and/or lipemia, depending on laboratory method and handling.

Technician (extrinsic) limitations

  • Generally accurate.
  • Routine assay.

Result Data

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Further Reading

Publications

Other sources of information

  • Kaneko J J, Harvey J Wand Bruss N L (eds) (1997) Clinical Biochemisrty of Domestic Animals.5th edition, Academic Press, Boston.

Organisation(s)

  • Veterinary commercial laboratories, State diagnostic laboratories or Veterinary College laboratories.


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