Equis ISSN 2398-2977

Blood: agglutination test - jaundiced foal

Contributor(s): Prof Derek Knottenbelt

Sampling

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Tests

Methodologies

Materials required
  • Centrifuge: 300-600 g - check revs for this rating.
  • Test tube rack and disposable sterile 10 ml test tubes - possibly centrifuge type.
  • Pasteur/automatic pipettes with 1 ml dispensation volume.
  • Isotonic saline at room temperature.
  • Red cells and serum/colostrum from mare - lithium heparin/citrated.
    Filter colostrum through gauze
  • Red cells from foal, in lithium heparin/citrated, pre-suckle.
    Method
  • Set up 7 tubes each containing 1 ml of saline.
  • Label tubes:
    • 1:2.
    • 1:4.
    • 1:8.
    • 1:16.
    • 1:32.
    • 1:64.
    • Blank.
  • Make serial dilutions of mares plasma/colostrum by adding 1 ml to tube 1 and mixing with a pipette, remove 1 ml from this tube into the next, mix, remove 1 ml to the third tube and so on. Discard 1 ml from the sixth tube - 1:64.
  • Add one drop of the foal's whole blood to each of the seven tubes and mix by agitation or mixer.
  • Centrifuge tubes for 2-3 min at 200-300 g.
  • Pour-off supernatant from each tube and observe the status of the red cell button.
    Do not let the foal suckle the mare
    Supply colostrum from other source

Result Data

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Further Reading

Publications

Other sources of information

  • Bailey E, Conboy H S & McCarthy P F (1987)Neonatal isoerythrolysis of foals - An update on testing.In: Proceedings of the Annual Congress of the AAEP. pp 123-132.


ADDED