ISSN 2398-2977      

Staining techniques: Wright's stain

pequis
Contributor(s):

Synonym(s): Romanowsky stain


Introduction

Rationale

Uses

  • Hematology and cytology.

Pros

  • If Hemofast is used, it contains neutral methyl alcohol, ie does not contain acetone, therefore the solution can fix itself and there is no need for prior fixing of the smeared slides.

Cons

  • Must use distilled water for Hemofast.
  • Cannot use tap water since the latter contains interfering substances, particularly calcium which can cause precipitation of eosin to an insoluble calcium salt.

Alternatives

Work time

  • 5-15 min depending on whether the stain has been prepared or is ready mixed.
  • Refer to methodology for details.

Requirements

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Method

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Result data

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Storage

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Errors

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Further Reading

Publications

Refereed papers

Other sources of information

  • Lewis H B & Rebar A H (1979) Bone Marrow Evaluation in Veterinary Practice. Ralston Purina Co. pp 59.

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