Equis ISSN 2398-2977
Staining techniques: Romanowsky stains - overview
Contributor(s): Annalisa Barrelet, Kathleen P Freeman, Karen L Gerber, T Scrivener, Malcolm J Wheeler
- Romanowsky stains are neutral stains composed of a mixture of oxidized methylene blue (azure) dyes and Eosin Y.
- The azures are basic dyes that bind acid nuclei and result in a blue to purple color.
- The acid dye, eosin, is attracted to the alkaline cytoplasm, producing red coloration.
- Blood films, bone marrow examination, cytology.
- Readily available, easy to prepare, maintain and use.
- Nuclear and nucleolar detail not as good as papanicolaou, but adequate to distinguish neoplasia from inflammation.
- Ph-sensitive therefore buffer and rinse water are critical (refer to artifacts/errors).
- Preparation: depends on type of Romanowsky stain - refer to sub-categories.
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Other sources of information
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- Pratt P W (1997) Laboratory Procedures For Veternary Technicians. 3rd edn. Mosby. pp 547.
- Bain B J (1989) Blood Cells - A Practical Guide. Gower Medical Publishing. pp 8-10.
- Hall R & Malia R (1984) Medical Laboratory Hematology. Butterworth & Co Ltd. pp 132-136.