Equis ISSN 2398-2977

Staining techniques: Modified Ziehl-Neelson stain

Synonym(s): MZN

Contributor(s): Tony Bacon, Annalisa Barrelet, Kathleen P Freeman, Karen L Gerber, T Scrivener, Malcolm J Wheeler

Introduction

Rationale
  • Stain bacteria that are partially acid-fast. These organisms can be decolorized by acid-alcohol and therefore 0.5% acetic acid is used as the decolorizer.

Uses

  • To identify acid-fast bacteria.

technique

  • Technician: must wear protective goggles when heating carbol fuschin.

Alternatives

  • Kinyoun (for Nocarida).

Work time

  • 15-25 min.

Preparation

  • 15-23 min to stain.

Procedure

  • 2 min microscope identification.

Requirements

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Method

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Result data

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Storage

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Further Reading

Publications

Refereed papers

Other sources of information

  • Pratt P W (1997) Laboratory Procedures for Veterinary Technicians. 3rd edn. Mosby. pp 174.
  • Barrow G I & Feltham R K A (1993) Cowan and Steel's Manual for the Identification of Medical Bacteria. 3rd edn. Cambridge University Press. pp 217.


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