Equis ISSN 2398-2977

Staining techniques: Diffquick stain

Synonym(s): Romanowsky, Wright's-type stain

Contributor(s): Annalisa Barrelet, Kathleen P Freeman, Karen L Gerber, T Scrivener, Malcolm J Wheeler

Introduction

Rationale
  • Diffquick is a Wrights-type rapid blood stain which is a modification of the original Fields stain (reverse order and weaker concentration).
  • Modified Diffquick protocol (alcohol fixed) results in excellent cell detail but lacks the Romanowsky effect, resulting in blue rather than metachromatic purple color.

Uses

  • Hematology - blood films.
  • Cytology.
  • Histology (Helicobacter).

Pros

  • A rapid staining technique.

Cons

  • The stain does not undergo a metachromatic reaction. Therefore it does not always stain basophil or mast cell granules, which is a particular disadvantage in cytology.
  • Can get variation in coloration with slight changes in staining times.

Alternatives

Work time

  • +/- 1 min.

Preparation

  • +/- 30 s.

Procedure

  • +/- 15 s.

Requirements

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Method

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Storage

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Errors

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Further Reading

Publications

Refereed papers


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