Equis ISSN 2398-2977

Endocrine: insulin response test

Synonym(s): Insulin tolerance test, Combined intravenous glucose and insulin test, Glucose tolerance test

Contributor(s): Janice Sojka, Ramiro Toribio, Han van der Kolk

Introduction

  • The insulin response test is a method to estimate insulin resistance in horses. 
  • Its use has fallen out of favor due to the risk of inducing symptomatic hypoglycemia in normal horses and the availability of other testing like minimal model analysis and the glucose clamp test. With reference to assessment of (peripheral) insulin resistance the so-called hyperinsulinemic euglycemic clamp test is regarded as 'gold standard'. 
  • The insulin response test has largely been replaced by the intravenous glucose tolerance test, the hyperinsulinemic euglycemic glucose clamp test or the glucose/insulin ratio assessed via minimal model analysis. 
  • A recently described combined glucose and insulin response test can also be used to document insulin resistance in horses.

Insulin resistance

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Glucose tolerance test

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Insulin response test (insulin stimulation test)

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Combined intravenous glucose and insulin test

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Expected results and interpretation

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Further Reading

Publications

Refereed papers
  • Recent references fromPubMedpublished during the last 12 months.
  • Toth F et al(2009)Optimisation of the frequently sampled intravenous glucose tolerance test to reduce urinary glucose spilling in horses. Equine Vet Educ41(9), 844-851.
  • Eiler H, Frank N, Andrews F, Oliver J W & Fecteau K A (2005)Physiologic assessment of blood glucose homeostasis via combined intravenous glucose and insulin testing in horses. AJVR66, 1598-1604PubMed.
  • Pratt S E, Geor R J & McCutcheon L J (2005) Repeatability of 2 methods for assessment of insulin sensitivity and glucose dynamics in horses. J Vet Intern Med19(6), 883-888PubMed.
  • Treiber K H, Kronfeld D S, Hess T M, Boston R C & Harris P A (2005) Use of proxies and reference quintiles obtained from minimal model analysis for determination of insulin sensitivity and pancreatic beta-cell responsiveness in horses. Am J Vet Res66(12), 2114-2121PubMed.
  • Rijnen K E & van der Kolk J H (2003) Determination of reference range values indicative of glucose metabolism and insulin resistance by use of glucose clamp techniques in horses and ponies. Am J Vet Res64(10), 1260-1264. Erratum in: Am J Vet Res2004 Mar;65(3), 362PubMed
  • Staempfli H R, Eigenmann E J & Clarke L M (1988) Insulin treatment and development of anti-insulin antibodies in a horse with diabetes mellitus associated with a functional pituitary adenoma. Can Vet J29, 934-936 PubMed.

Other sources of information

  • Toribio R E (2004)Endocrine Pancreas. In: Equine Internal Medicine. Eds: Reed S M, Bayly W M, & Sellon D C. 2nd edn. Saunders, St Louis. pp 1362-1365.
  • Beech J (1999)diseases of the Pituitary Gland.In: Equine Medicine & Surgery. Eds: Colahan P T, Mayhew I G, Merritt A M & Moore J N. 5th edn. Mosby Inc, St Louis, MO. pp 1951-1956.


ADDED