ISSN 2398-2942      

Indirect Coombs' test

icanis
Contributor(s):

Vetstream Ltd

Synonym(s): Indirect antiglobulin test


Overview

  • Theoretically detects presence of antibodies against erythrocytes in the plasma or serum.
  • Low sensitivity compared to direct Coombs' test Direct Coombs' test , therefore only used if sample of cells unavailable.

Sampling

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Tests

Methodologies

  • Plasma or serum sample.
  • Detects free antibodies directed against patient's erythrocytes.

Availability

  • Rarely carried out.

Validity

Sensitivity

Specificity

  • Susceptible to false positives.

Technique (intrinsic) limitations

  • Low sensitivity of detection of antibodies.
  • Cannot provide definitive diagnosis of auto-immune or immune-mediated hemolytic anemia even if positive.

Technician (extrinsic) limitations

  • Very low sensitivity.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMedResource and PubMed.
  • Day M J (1996) Serial monitoring of clinical, hematological and immunological parameters in canine autoimmune hemolytic anemia. JSAP 37 (11), 523-534.
  • Jones D R et al (1987) An enzyme-linked antiglobulin test for the detection of erythrocyte-bounc antibodies in canine autoimmune hemolytic anemia. Vet immunol Immunopathol 16 (1-2), 11-21.

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