Canis ISSN: 2398-2942

Canine pancreatic lipase immunoreactivity (cPLI) test

Contributor(s): Craig Ruaux, Joerg Steiner

Overview

  • cPLI is an acronym for canine Pancreatic Lipase Immunoreactivity, a specific lipase found only in canine pancreatic tissue.
  • The commercially available assay (Spec cPL" by Idexx Laboratories) measures concentrations of pancreatic lipase in the serum.
  • Inflammation of the pancreas leads to an increased release of pancreatic lipase into the circulation, thus elevated concentrations of pancreatic lipase are consistent with a diagnosis of pancreatitis Pancreatitis: acute Pancreatitis: chronic.

Sampling

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Tests

Methodologies

  • Serum canine pancreatic lipase immunoreactivity is measured with an ELISA Enzyme linked immunosorbent assay (ELISA).
  • This test (Spec cPL") is exclusively manufactured by Idexx Laboratories.
    • A microplate is coated with monoclonal antibodies against canine pancreatic lipase.
    • Sera are diluted and incubated on the plate, allowing the monoclonal antibodies to capture the pancreatic lipase, while other proteins are washed away.
    • A secondary antibody carrying a label is then incubated in the wells.
    • The amount of bound label is proportionate to the quantity of pancreatic lipase in the original serum sample.
    • A standard curve is constructed using a recombinant peptide that is part of the native canine pancreatic lipase protein.

Availability

  • Serum canine pancreatic lipase immunoreactivity measurement (Spec cPL" Assay) is currently only available via an Idexx Reference Laboratory or the Gastrointestinal Laboratory at Texas A&M University.

Validity

Sensitivity

  • Determination of canine pancreatic lipase in serum has a sensitivity of 82% for the diagnosis of pancreatitis.

Specificity

  • Determination of canine pancreatic lipase generally has >95% specificity for the diagnosis of pancreatitis. Specificities of 100% have been reported in some studies.

Technique (intrinsic) limitations

  • Avoid submission of severely lipemic or hemolyzed samples.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMed Resource and PubMed.
  • Steiner J M., Rutz G M & Williams D A (2006) Serum lipase activities and pancreatic lipase immunoreactivity concentrations in dogs with exocrine pancreatic insufficiency. Am J Vet Res 67(1), 84-7 PubMed.
  • Steiner J M, Broussard J, Mansfield C S, Gumminger S R & Williams D A (2001) Serum canine pancreatic lipase immunoreactivity (cPLI) concentrations in dogs with spontaneous pancreatitis. J Vet Intern Med 15, 274.
  • Steiner J M, Finco D R, Gumminger S R & Williams D A (2001) Serum canine pancreatic lipase immunoreactivity (cPLI) in dogs with experimentally induced chronic renal failure. J Vet Intern Med 15, 311.

Other sources of information

  • Mansfield C (2004) New directions in diagnosing and treating canine pancreatitis. Proceedings of the ACVIM Forum 2004, Minneapolis, June 912, 2004.

Organisation(s)


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