Canis ISSN: 2398-2942

Anti-nuclear antibody

Synonym(s): SLE test, ANA test

Contributor(s): Vetstream Ltd

Overview

  • In immune-mediated disease; antibodies directed against cell nucleus, detected by agglutination of coated latex particles or indirect immunofluorescence.

Sampling

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Tests

Methodologies

Indirect immunofluorescence

  • Dilutions of patient's serum incubated with mouse/rat liver or tissue culture cells.
  • If present, anti-nuclear antibodies bind to substrate.
  • ANA detected by fluorescein-conjugated anti-canine globulin.
  • Titer: highest dilution of patient's serum causing staining of nuclei.
  • Positive titer depends on cell substrate; usually 1:40 to 1:100.

Latex agglutination

  • Agglutination of coated latex particles by ANA.
  • Quantitates anti-nucleoprotein serum factors.
  • Commercial kit available.

Validity

Sensitivity

  • High, especially with tissue culture immunoflourescent test.

Specificity

  • Poor since many other diseases may have ANA titer; usually lower than in SLE.

Predictive value

  • Not reported.

Technique (intrinsic) limitations

  • Absolute diagnosis of SLE requires a positive ANA test and at least two major clinical signs of SLE.

Technician (extrinsic) limitations

  • Reading of immunofluorescence results requires experience.
  • Normal values vary between laboratories.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMed Resource and PubMed.
  • Jones D R (1993) Canine systemic lupus erythematosus - new insights and their implications. J Comp Pathol 108(3), 215-228.
  • Monier J C (1992) Canine systemic lupus erythematosus. II Antinuclear antibodies. Lupus 1(5), 287-293.
  • McVey D S (1991) Use of multiple antigen substrates to detect antinuclear antibody in canine sera. Vet Immunol Immunopathol 28(1), 37-43.


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