Bovis ISSN 2398-2993

Staining techniques: glossary

Contributor(s): Vetstream Ltd

Equipment

Cuvet (cuvette)

  • A plastic or glass tube, circular or square in cross-section and sealed at one end.
  • Used for holding samples for spectroscopic analysis.

Bunsen burner

  • A single open gas flame or heating, sterilization and combustion.

Pipette

  • Laboratory tool used for transporting a measured amount of liquid.

Slides

  • A thin flat piece of glass, occasionally plastic, that acts as a surface for samples to be spread on for examination using a microscope.

Cover slip

  • A very small thin piece of glass that is placed over the top of a biological sample on a microscope slide to protect the sample and fix it in place.

Adhesive covered slides

  • Microscope slides that have had adhesive sprayed on in order to improve the adherence of cells (usually) to the slide.
  • Adhesives include poly-l-lysine, epoxy resin or gold.

Cytospin slide

  • The 'cytospin' is a type of centrifuge.
  • It can be used to funnel cells from suspension onto microscope slides (hence a 'cytospin slide').
  • The samples produces have good agreement with direct smears.

Coplin jar

  • A glass jar (often with a heavy base and glass lid) that is used to contain staining chemicals. 
  • May be clear or dark glass (depending on the chemical to be contained) and may have internal slots to allow several slides to be stained at once.

Staining rack

  • Metal rack that will hold several microscope slides allowing more than one slide to be stained at one time.

Slide dryer

  • A flat rack with a heating element, on which stained slides can be placed to dry before being examined.

Smear fix

  • Chemical substance that stabilized the proteins, nucleic acids and mucosubstances in the cell or sample making them inert and allowing preservation in a state as near to natural as possible.

Chemicals and dyes

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Terminology

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Further Reading

Publications

Refereed Papers


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