Lapis ISSN 2398-2969

Blood biochemistry: total protein

Synonym(s): TP

Contributor(s): Anna Meredith, Vetstream Ltd, Michael Waters, John Chitty

Overview

  • Comprises albumin and globulin fractions.
  • Globulin fraction = alpha, beta and gamma subfractions.
  • Absolute changes in albumin and globulin associated with wide range of clinical conditions.
  • Electrophoresis: separation of globulin subfractions provides further information.

Sampling

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Tests

Methodologies

  • Biuret: standard method.
  • Calibrated refractometer: for rapid result.
  • Most accurate results using plasma rather than serum.
  • Electrophoresis: followed by scanning with densitometer to quantify individual globulin subfractions.

Availability

  • Biuret: widely available.
  • Refractometer: widely available.
  • Electrophoresis: commercial laboratories.

Validity

Sensitivity

  • Biuret: accurate except at low levels (<10 g/l).
  • Refractometer: generally satisfactory for clinical purposes.

Technique (intrinsic) limitations

  • Usually must be interpreted in conjunction with other laboratory and clinical findings to reach a diagnosis.

Technician (extrinsic) limitations

  • Electrophoresis.
  • Biuret.
  • Refractometer.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from PubMed and VetMedResource.
  • Benson K G & Paul-Murphy J (1999) Clinical pathology of the domestic rabbit: acquisition and interpretation of samples. Vet Clin North Am Exotic Anim Pract 2 (3), 539-552 PubMed.

Other sources of information

  • Varga M (2014) Textbook of Rabbit Medicine. 2nd edn. Butterworth Heinemann Elsevier, London, UK.
  • Wesche P (2014) Clinical Pathology. In: BSAVA Manual of Rabbit Medicine. Eds: Meredith A & Lord B. pp 124-137. BSAVA, Gloucester, UK.
  • Saunders R A & Rees Davies R (2005) Notes on Rabbit Internal Medicine. Blackwells, Oxford, UK.


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