Felis ISSN 2398-2950

Staining techniques: Leishman's stain

Contributor(s): Kathleen P Freeman, Karen Gerber


  • A neutral stain for blood smears devised by a British surgeon,W B Leishman (1865-1926).
  • Double staining compound of Methylene blue and Eosin in alcohol, which is usually diluted and buffered before use.
  • It stains the different components of blood in a range of shades between red and blue.


  • Stains WBC, RBC and platelets.


  • Referral to laboratory or human medical institution.


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Further Reading


Refereed papers

Other sources of information

  • Dacia Sir J V &  Lewis S M (2001) Practical Haematology. 6th edn, Longman Singapore publishers Pte Ltd, pp 50-61.
  • Bain B J (1989) Blood Cells, A Practical Guide. Gower Medical Publishing,  pp 810.
  • Hall R & Malia R (1984) Medical Laboratory Haematology. Butterworth & Co (publishers) Ltd,  pp 132-136.